Genetic Variability of Nile Tilapia Strains as Determined by Microsatellite DNA Markers

The main purpose of the present study was to investigate genetic variability Nile Tilapia Oreochromis niloticus strains by using microsatellite DNA markers and compare these data with those previously obtained single-nucleotide polymorphisms (SNPs). Five mixed-sex four YY-male were analyzed. All nine loci polymorphic; number unique alleles per locus varied from 6 at UNH216 33 UNH880. Strong correspondence observed between level expected heterozygosity (He) or (A) private alleles. Strains high levels typically had higher values A presence In general, than strains. Four (GIFT, Ismailia Canal, LSA hybrid, Miami) highest variability, mean He ranging 0.630 0.798 5.11 9.78. Three (Fishgen YY Dark–Group 2, Fishgen Red, Til-Aqua YY) low 0.282 0.367 1.89 3.56. investigated demonstrated inbreeding coefficient FIS (from –0.266 0.014), suggesting that mating closely related individuals is being avoided during maintenance Pairwise differentiation index FST a broad range 0.0915 0.6063), indicating different dendrogram constructed according shared allele distance showed existence two distinct clusters among on substantial similarity previous SNPs in same populations.